{
"@context": "/terms/",
"@id": "/tabular-files/IGVFFI0477HTCF/",
"@type": [
"TabularFile",
"File",
"Item"
],
"accession": "IGVFFI0477HTCF",
"aliases": [
"charles-gersbach:cd8-tcell-cd2-grnas-metadata-v5"
],
"assay_titles": [],
"assembly": "GRCh38",
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released tabular file [IGVFFI0477HTCF](/tabular-files/IGVFFI0477HTCF/) has in progress subobject curated set [IGVFDS1006FHCX](/curated-sets/IGVFDS1006FHCX/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/tabular-files/IGVFFI0477HTCF/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"barcode_map_for": [],
"checkfiles_version": "v20",
"content_md5sum": "680d0b6ce2a229e2ded3516a3eff9d94",
"content_type": "guide RNA sequences",
"controlled_access": false,
"creation_timestamp": "2025-03-21T03:18:06.061448+00:00",
"file_format": "tsv",
"file_format_specifications": [
"/documents/78670549-3719-42bc-8df2-92d98918fa27/"
],
"file_set": {
"@id": "/curated-sets/IGVFDS1006FHCX/",
"@type": [
"CuratedSet",
"FileSet",
"Item"
],
"accession": "IGVFDS1006FHCX",
"file_set_type": "guide RNAs",
"status": "in progress",
"summary": "Homo sapiens GRCh38 custom guide RNAs",
"taxa": "Homo sapiens"
},
"file_size": 5789,
"gene_list_for": [],
"href": "/tabular-files/IGVFFI0477HTCF/@@download/IGVFFI0477HTCF.tsv.gz",
"input_file_for": [],
"integrated_in": [
{
"@id": "/construct-library-sets/IGVFDS8345ZMZW/",
"applied_to_samples": [
{
"@id": "/primary-cells/IGVFSM1730FNFX/",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM3002NRDK/",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM4694MBIW/",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7030KVXG/",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM6350DJQS/",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM7658WDNM/",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"summary": "CD8-positive, alpha-beta memory T cell",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)"
}
],
"file_set_type": "guide library",
"small_scale_gene_list": [
{
"@id": "/genes/ENSG00000116824/",
"status": "released",
"symbol": "CD2"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting transcription start sites in CD2"
}
],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "37942b1a33fcd6ab0369dce9bf9559c6",
"primer_design_for": [],
"quality_metrics": [],
"release_timestamp": "2025-04-25T03:34:37.063964+00:00",
"s3_uri": "s3://igvf-files/2025/03/21/38636da1-8392-4fa5-8170-407ae4d1a4a3/IGVFFI0477HTCF.tsv.gz",
"schema_version": "15",
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CD2.screen.igvf_metadata.v6.txt.gz",
"summary": "GRCh38 guide RNA sequences",
"upload_status": "validated",
"uuid": "38636da1-8392-4fa5-8170-407ae4d1a4a3"
}