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IGVFFI0420XBKB

released
validated
File Set
IGVFDS9143TFRW(CRISPR interference FACS screen targeting CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes with low FACS signal control)
Summary
custom assembly fold change over control
Analysis Step Version
/analysis-step-versions/6a1d20c7-c92f-47d6-8e3b-5c74b3e8eed2/
File Format
tsv
Content Type
fold change over control
Aliases
charles-gersbach:cd8-tcells-guide-quantifications-crispri-ccr7
md5sum
a524709086ecef977ee8f9b28cf78f93
Content MD5sum
447a3eea06a73765790066fb70692824
File Size
70 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/T2C_DESeq2_CRISPRi_TF_screen.simple.v2.txt.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Charles Gersbach, Duke

Reference Source Details

Genome Assembly
custom

File Format Specifications

1 item
Page
Description
Type
Download
Standardized File Format
Preview
DESeq2 results
file format specification

Samples

4 items
Sample Terms
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
csv
guide quantifications
Charles Gersbach, Duke
40 KB
validated