IGVFFI0420XBKB

released
validated
File Set
IGVFDS9143TFRW(CRISPR interference FACS screen targeting CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
custom assembly fold change over control
Analysis Step Version
File Format
tsv
Content Type
fold change over control
Aliases
charles-gersbach:cd8-tcells-guide-quantifications-crispri-ccr7
md5sum
a524709086ecef977ee8f9b28cf78f93
Content MD5sum
447a3eea06a73765790066fb70692824
File Size
70 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/T2C_DESeq2_CRISPRi_TF_screen.simple.v2.txt.gz
Checkfiles Version
Attribution

Reference Source Details

Genome Assembly
custom

File Format Specifications

1 item

Samples

4 items
Sample Terms
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
csv
guide quantifications
Charles Gersbach, Duke
40 KB
validated