IGVFFI9973DZYW

released

validated

File Set

IGVFDS7007ZSGW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR1-Rep1_S37

md5sum

9698d5545d1d7c27e4cfff994710ea4a

Content MD5sum

25ddeb59e1c558595c3ea5f7944be70d

File Size

7.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF2-BinA-PCR1-Rep1_S37_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AAGACACT:TTGA

Average Read Length

Read Count

359517

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2476YZDG

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library