IGVFFI9919VFXO

released
validated
File Set
IGVFDS6678UHIV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinD-PCR2_Rep1_S144
md5sum
5d596d56c49b194e4cc37f658f37568c
Content MD5sum
f0d0f416b879919a97df3425644a7afc
File Size
24 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinD-PCR2-Rep1_S144_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Index
GTCTGATG:TTGA
Average Read Length
21
Read Count
1230820

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI9919VFXO
Jesse Engreitz, Stanford
913 B
validated

Attribution