IGVFFI9918BQUQ

released

validated

File Set

IGVFDS8524AJUL(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF3-BinE-PCR1_S77

md5sum

ce3e7b7830eb08bbb2725b190c1767f1

Content MD5sum

431f7ba66b86b7f7c3198ceebd153783

File Size

5.7 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF3-BinE-PCR1_S77_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ACAGTTGA:CTCT

Average Read Length

Read Count

237307

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9250VIBU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library