IGVFFI9916XQDH

released
validated
File Set
IGVFDS0591VNKJ(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
R1 reads from sequencing run 3
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep3-rep3-fastq-lane1-R1
md5sum
9d83201c668b7903d9220320b32345e6
Content MD5sum
a41e97641ff1ecb8671c8762d6610c1c
File Size
2.3 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/gRNA_CRISPRi_D3_S9_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
3
Flowcell ID
HMCCWDSX3
Lane
1
Sequencing Kit
NovaSeq 6000 S4 Reagent Kit v1.5
Illumina Read Type
R1
Average Read Length
26
Read Count
114208719

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
335 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI9916XQDH, IGVFFI2856WURS
Charles Gersbach, Duke
1.1 KB
validated

Attribution