{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI9851LYSX/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI9851LYSX",
"aliases": [
"jesse-engreitz:sequence_files-TeloHAEC_Perturb-seq_2kG-Dialout_2kg_2PMDox1_5.2_CGTTACCA_L001_R2_001.fastq.gz"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "b8ac4d25119951be4aa6c2fdc1217f83",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-02-06T21:22:13.031584+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS0616JFOF/",
"accession": "IGVFDS0616JFOF",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM1061NWCP/",
"accession": "IGVFSM1061NWCP",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"status": "released",
"term_name": "TeloHAEC"
}
],
"status": "preview",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci"
},
"file_size": 420480969,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI9851LYSX/@@download/IGVFFI9851LYSX.fastq.gz",
"illumina_read_type": "R2",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 60,
"md5sum": "0d7954ded17cb884c0f546d4bba67d18",
"mean_read_length": 60,
"minimum_read_length": 60,
"read_count": 19661377,
"s3_uri": "s3://igvf-files/2024/02/06/b817442b-c889-47aa-81ad-abb403080bdb/IGVFFI9851LYSX.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "NextSeq 500/550 High-Output v2.5 Kit",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0009173/",
"status": "released",
"term_name": "Illumina NextSeq 500"
},
"sequencing_run": 2,
"status": "preview",
"submitted_by": {
"@id": "/users/05fa0dc9-0e33-427c-9d1f-82393edc4a03/",
"title": "Helen Kang"
},
"submitted_file_name": "/oak/stanford/groups/engreitz/Users/gschnitz/dialout_files_for_helen/Dialout_2kg_2PMDox1_5.2_CGTTACCA_L001_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 2",
"upload_status": "validated",
"uuid": "b817442b-c889-47aa-81ad-abb403080bdb"
}