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IGVFFI9800CRJB

released
validated
File Set
IGVFDS0667KDIQ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep1-FFrep2-BinE-PCRrep1_I1
md5sum
c6cd5fec6fa362b9bafe24ed16828bc9
Content MD5sum
3f51dcb2c6aec0c81e10bc09b743baea
File Size
759 KB
Submitted File Name
220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S3-BioRep1-FFRep2-BinE-PCRrep1_S12_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
AGGTCGCA:CAGT
Average Read Length
8
Read Count
90130

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford