IGVFFI9695GFTP

released

validated

File Set

IGVFDS1380VYWN(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF1-BinB-PCR4-Rep1_S86

md5sum

c2b379f9a03784f085bd2a19067d94d1

Content MD5sum

1d0cba57a8b8222c201800f9b5d985d9

File Size

20 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF1-BinB-PCR4-Rep1_S86_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCTAGG:GCCA

Average Read Length

Read Count

927562

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8531AKQD

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library