IGVFFI9642HYKU

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI9642HYKU/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI9642HYKU",
    "aliases": [
        "guillaume-lettre:BP_screen_KRAB_L_R4_1_read2"
    ],
    "assay_titles": [
        "CRISPR FlowFISH screen"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012010/",
        "component": "functional characterization"
    },
    "content_md5sum": "1225ec043ed96d02bf655c2f59624f86",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-11-15T21:29:10.793742+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/measurement-sets/IGVFDS1395QABS/",
        "accession": "IGVFDS1395QABS",
        "file_set_type": "experimental data",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM0340TUAL/",
                "accession": "IGVFSM0340TUAL",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/dca41650-4b6d-4685-b9c0-6c003f2d872a/",
                        "modality": "interference",
                        "status": "in progress",
                        "summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/NTR_0000753/",
                        "status": "released",
                        "term_name": "TeloHAEC"
                    }
                ],
                "status": "released",
                "summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "released",
        "summary": "CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure"
    },
    "file_size": 2356392724,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI9642HYKU/@@download/IGVFFI9642HYKU.fastq.gz",
    "illumina_read_type": "R2",
    "input_file_for": [
        "/tabular-files/IGVFFI3105FTIJ/",
        "/tabular-files/IGVFFI4455WXXM/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/guillaume-lettre/",
        "title": "Guillaume Lettre, MHI"
    },
    "loci_list_for": [],
    "maximum_read_length": 151,
    "md5sum": "2c35e258fa96ccde61f76ffa413e2912",
    "mean_read_length": 151,
    "minimum_read_length": 151,
    "quality_metrics": [],
    "read_count": 42020693,
    "release_timestamp": "2025-03-07T23:05:36.231837+00:00",
    "s3_uri": "s3://igvf-files/2024/11/15/b5d57278-dc1c-4925-8fe2-a019ed224db8/IGVFFI9642HYKU.fastq.gz",
    "schema_version": "15",
    "seqspecs": [],
    "sequencing_kit": "NovaSeq 6000 SP Reagent Kit v1.5",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0008637/",
        "status": "released",
        "term_name": "Illumina NovaSeq 6000"
    },
    "sequencing_run": 1,
    "status": "released",
    "submitted_by": {
        "@id": "/users/005a9a1c-02ab-408f-90a9-d6bea5c36d05/",
        "title": "Ken Sin Lo"
    },
    "submitted_file_name": "/home/lokensin/projects/rrg-glettre/sequencing_datastore/raw_data/CRISPR_IGVF_20230720/samples/NS.X0085.005.IndexR1_22.1BPR4_L_R2.fastq.gz",
    "summary": "R2 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "b5d57278-dc1c-4925-8fe2-a019ed224db8"
}