IGVFFI9605MXIW

released

validated

File Set

IGVFDS3199FLOT(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR3_S97

md5sum

c3b9923da7b1b80701c5fa2e7b3cf3b5

Content MD5sum

f087c2b1aff7fae8f07d04048d92bd30

File Size

36 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF1-BinA-PCR3_S97_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CTACCAGG:ATTG

Average Read Length

Read Count

1525056

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8111MLZW

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library