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IGVFFI9511EKXQ

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1128FXRR(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW286B_1_I2
md5sum
933f379283fabfb50e714d31860fa959
Content MD5sum
2761e83753c920cb3bb1bd91fcb6bf79
File Size
394 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-11-03-McDermott-NextSeq.10X.run2.10X/2023-11-03/outs/fastq_path/AAF3JCVM5//LW286B_S1_L001_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAF3JCVM5
Lane
1
Illumina Read Type
I2
Average Read Length
10
Read Count
66075545

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW286) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
589 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
429 MB
validated