IGVFFI9434BMBU

released

validated

File Set

IGVFDS0133OAZC(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF1-BinA-PCR1-Rep1_S25

md5sum

77e1f31b60b2eb0046511f232077cc24

Content MD5sum

c8c48f6de59d1f9e941121529a5ab3ef

File Size

15 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF1-BinA-PCR1-Rep1_S25_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GAAGAAGT:TTGA

Average Read Length

Read Count

710621

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8298SPUC

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library