IGVFFI9405VYLM

released
validated
File Set
IGVFDS5686BCWV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR1-Rep1_S16
md5sum
2f7ac71df9015c76d1cce3f387c66f98
Content MD5sum
3fc72bd6995c31961b33cd463f3d3530
File Size
9.9 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF2-BinD-PCR1-Rep1_S16_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Index
AAGGATGT:TTGA
Average Read Length
21
Read Count
453033

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI9405VYLM
Jesse Engreitz, Stanford
914 B
validated

Attribution