IGVFFI9349ZEJD

released

validated

File Set

IGVFDS9693EKGR(gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispri-ccr7-low-bin-rep1-R1

md5sum

64f2d11a544dfd6b6ae560174c0174ef

Content MD5sum

cada5e87727f4920e0bd183f87c1db67

File Size

51 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/CCR7pos-pSM27-TF-L1_S1_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

000000000-K4RT4

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

2150550

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7413JCSK

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)