IGVFFI9320EENZ

released

validated

File Set

IGVFDS7634SPOA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep1_S10

md5sum

d40265f9c565e65e917b1fe53336aaa7

Content MD5sum

0089a71a4432792acc3fc1baa4dd7671

File Size

29 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinD-PCR2-Rep1_S10_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GGTCCAGA:TGCG

Average Read Length

Read Count

1487693

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0111NTPA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library