IGVFFI9302YGJM

released
validated
File Set
IGVFDS1872APYF(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinC-PCRrep2_I1
md5sum
b4ae46260a424d289fb2759fc7096169
Content MD5sum
b9dc0b7a2c7b8c4ae8d03294092f2606
File Size
3.3 MB
Submitted File Name
220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinC-PCRrep2_S193_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I1
Index
AGGTGCGA:ACTG
Average Read Length
8
Read Count
425711

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution