IGVFFI9284TWQH

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI9284TWQH/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI9284TWQH",
    "aliases": [
        "charles-gersbach:measurement-set-crispra-control-1umET1-ipsc-cm-targeted-hicar-rep1-R1"
    ],
    "assay_titles": [
        "HiCAR"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "da1211028193dd12ec84212b30beffdc",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-10-23T19:29:34.437580+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/measurement-sets/IGVFDS2114RIWO/",
        "accession": "IGVFDS2114RIWO",
        "file_set_type": "experimental data",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM2698DFOT/",
                "accession": "IGVFSM2698DFOT",
                "classifications": [
                    "differentiated cell specimen"
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0009747/",
                        "status": "released",
                        "term_name": "GM25256 (WTC-11)"
                    }
                ],
                "status": "released",
                "summary": "GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 42 days, male, Homo sapiens treated with 1 μM Endothelin-1 for 3 days, transfected with a guide library",
                "targeted_sample_term": {
                    "@id": "/sample-terms/CL_0000746/",
                    "status": "released",
                    "term_name": "cardiac muscle cell"
                },
                "taxa": "Homo sapiens",
                "treatments": [
                    {
                        "@id": "/treatments/bbe5df8d-a9e2-4a99-8942-9b3b7d12c605/",
                        "purpose": "perturbation",
                        "status": "released",
                        "summary": "Treatment of 1 μM Endothelin-1 for 3 days",
                        "treatment_term_name": "Endothelin-1"
                    }
                ]
            }
        ],
        "status": "released",
        "summary": "CRISPR HiCAR integrating a non-targeting guide (sgRNA) library"
    },
    "file_size": 1036199526,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI9284TWQH/@@download/IGVFFI9284TWQH.fastq.gz",
    "illumina_read_type": "R1",
    "input_file_for": [
        "/matrix-files/IGVFFI1058WSVT/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "maximum_read_length": 100,
    "md5sum": "bd160d10f85fbab3fe759ba0efaa7fe3",
    "mean_read_length": 100,
    "minimum_read_length": 100,
    "read_count": 20412121,
    "release_timestamp": "2024-12-13T19:43:54.745352+00:00",
    "s3_uri": "s3://igvf-files/2024/10/23/1a67eb86-c950-4a7f-b692-cc3876741f59/IGVFFI9284TWQH.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_kit": "NextSeq 1000/2000 P2 Reagent Kit",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0010963/",
        "status": "released",
        "term_name": "Illumina NextSeq 2000"
    },
    "sequencing_run": 4,
    "status": "released",
    "submitted_by": {
        "@id": "/users/3782ce69-0e9f-4b46-91f9-4578139785c7/",
        "title": "Ruhi Rai"
    },
    "submitted_file_name": "/hpc/group/gersbachlab/tda17/IGVF_data_files/HiCAR/fastq/crispra_human/crispra_hicar_human_fastq/H_vp64_r2_NT_1_1uMET1_ds.6a7891929f354459a698439ff23cadd2/H_vp64_r2_NT_1_1uMET1_S6_L001_R1_001.fastq.gz",
    "summary": "R1 reads from sequencing run 4",
    "upload_status": "validated",
    "uuid": "1a67eb86-c950-4a7f-b692-cc3876741f59"
}