IGVFFI9279HEMN

released

validated

File Set

IGVFDS4259ETEA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR1-Rep1_S222

md5sum

9c3397faab6cc843d6d752c4715a9102

Content MD5sum

c8edb1ce50e71fd0ee918bd288b68cef

File Size

19 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF1-BinF-PCR1-Rep1_S222_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAGCGGTA:TTGA

Average Read Length

Read Count

880707

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4218VHTT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library