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IGVFFI9244QRVR

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS8001EMIV(cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW266C_1_I2
md5sum
ad47bd5fc36907d6f3ec848d24420856
Content MD5sum
c96262bad1e0a5079fa3b803df284964
File Size
163 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-07-07-McDermott-NextSeq.10X.run1.sgRNA_HTO/2023-07-07/outs/fastq_path/AACV7CFM5/LW266C_S14_L001_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AACV7CFM5
Lane
1
Illumina Read Type
I2
Average Read Length
10
Read Count
20404093

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
5.2 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
443 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT