IGVFFI9220ZDKH

released

validated

File Set

IGVFDS6660JNZU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR3_Rep2_S207

md5sum

18a48cedb1d6e314916ef301e11ce864

Content MD5sum

1663c57bb63120d66cf28be8052caa19

File Size

28 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinF-PCR3-Rep2_S207_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGGTTATC:GCCA

Average Read Length

Read Count

1456528

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3012RMVA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library