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IGVFFI9175PZYF

released
validated
File Set
IGVFDS0595OBOA(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinA-PCRrep6_R2
md5sum
8b778777e5570d0a3d6d567e081776e4
Content MD5sum
db7b5cd1f2f830b64d047fa95a44d6f8
File Size
13 MB
Submitted File Name
230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep4_BinA-PCRrep6_S150_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
TCTCGGTC:GTCA
Average Read Length
150
Read Count
293225

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
hg19 variant effects
Jesse Engreitz, Stanford
11 KB
validated