IGVFFI9172PREQ

released

validated

File Set

IGVFDS2834VAYS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99

md5sum

5d0eb8969644ad44bbdb589243705200

Content MD5sum

75b0c4efac1b0b512954444c1a6c2734

File Size

12 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR4-Rep2_S99_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTTCGCA:TCTC

Average Read Length

Read Count

624387

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2313EONT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library