{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI9145LELN/",
"@type": [
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"File",
"Item"
],
"accession": "IGVFFI9145LELN",
"aliases": [
"gary-hon:LW431B_6_I1"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
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"component": "functional characterization"
},
"content_md5sum": "3118d81f587346c9e84f498886cfdc3a",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-11-13T19:43:05.302696+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
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"accession": "IGVFDS1182KBMU",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM5829AVCL/",
"accession": "IGVFSM5829AVCL",
"classifications": [
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],
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{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
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"summary": "CRISPRi Sp-dCas9"
}
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{
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"status": "released",
"term_name": "H9"
}
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"summary": "H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW431), female, Homo sapiens modified with CRISPRi Sp-dCas9, transfected with a guide library",
"targeted_sample_term": {
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"status": "released",
"term_name": "cardiac muscle cell"
},
"taxa": "Homo sapiens"
}
],
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"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
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"flowcell_id": "227C7WLT4",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI9145LELN/@@download/IGVFFI9145LELN.fastq.gz",
"illumina_read_type": "I1",
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"lab": {
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"title": "Gary Hon, UT"
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"lane": 6,
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"md5sum": "f2b4745a70d2c5044210a8bfabd20239",
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"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "NovaSeq X Series 25B Reagent Kit",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008637/",
"status": "released",
"term_name": "Illumina NovaSeq 6000"
},
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"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW431B_S5_L006_I1_001.fastq.gz",
"summary": "I1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "52adf5bc-b32b-411d-8ce8-4b1d5e67d23e"
}