IGVFFI9082ISDE

released

validated

File Set

IGVFDS0993NTCB(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinE-PCR4-Rep1_S185

md5sum

371e314f5d64221bd806a3fd3bb9d8d4

Content MD5sum

35cfd01cfd0879d932df74872ffb9c51

File Size

30 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinE-PCR4-Rep1_S185_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTACGCAC:ATTG

Average Read Length

Read Count

1394069

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1344KAZA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library