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IGVFFI9058JPJY

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1776XLCQ(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_CAAC-THP1-BioRep1-FFrep1-BinA-PCRrep4_I1
md5sum
d124727541d922d9b68b453058d8faee
Content MD5sum
cffda4bec76df43054e4495b7dde05a5
File Size
6.3 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C11-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinA-PCRrep4_S859_I1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
I1
Index
CCAGAGCT:CGCAGACG
Average Read Length
8
Read Count
843231

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
hg19 variant effects
Jesse Engreitz, Stanford
268 B
validated