IGVFFI9049KFCN

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1417TPKZ(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW437B_6_R2
md5sum
bacf02376c410784a7732b29cae8eee6
Content MD5sum
77f9fa010073e8db17c890ef7eacdeb5
File Size
5.6 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW437B_S11_L006_R2_001.fastq.gz
Checkfiles Version
Attribution

Sequencing Details

Sequencing Platform
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
6
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
R2
Average Read Length
151
Read Count
103091512

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW437) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Files Derived From This File

2 items
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
674 MB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
2.0 GB
validated