IGVFFI9023FXEP

released

validated

File Set

IGVFDS2426AOQY(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinA-PCR1-Rep1_S1

md5sum

27c976fb43a7064e7f55ac93b1f279e9

Content MD5sum

4befb55b33f2c4ff4cb45642a477bbdd

File Size

34 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinA-PCR1-Rep1_S1_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CTACCAGG:TTGA

Average Read Length

Read Count

1592104

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7231THBU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library