IGVFFI8999KUTF

released

validated

File Set

IGVFDS8375BGSZ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR1_S74

md5sum

1b86366a6d8bc06f0b91314d5248654f

Content MD5sum

5f435dc881ece42ecedc94dc8e7699fb

File Size

4.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinB-PCR1_S74_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCCTCT:ACGT

Average Read Length

Read Count

208941

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7863HHJP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library