IGVFFI8964HZGC

released

validated

File Set

IGVFDS8736DPTS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinC-PCR2-Rep1_S237

md5sum

842179c57257e2b49511ab0673bd7bec

Content MD5sum

3694afabf48a14b1624a017c351a41e1

File Size

21 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinC-PCR2-Rep1_S237_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTATCAA:TTGA

Average Read Length

Read Count

948280

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2887QEDU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library