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IGVFFI8810QOML

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS9847QETL(CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinC-PCRRep2_I2
md5sum
11c0b4ce48e3521fc8b47c1e4ab2bcab
Content MD5sum
a1f7bec42a41e4003f63dfa56837dd31
File Size
1.6 MB
Submitted File Name
Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-PCRRep2-BinC_S35_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
I2
Index
GACCTAAC:TTGT
Average Read Length
4
Read Count
206556

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 51-75% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
hg19 variant effects
Jesse Engreitz, Stanford
5.9 KB
validated