IGVFFI8734SFUZ

released

validated

File Set

IGVFDS5581ABLR(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep2_S60

md5sum

9b8ab03b86f2a0d67b20b9ac514b1568

Content MD5sum

656ac643fb679bbc07a38bbda5e83d78

File Size

31 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinE-PCR1-Rep2_S60_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGCAATTC:TCTC

Average Read Length

Read Count

1590397

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7084WNRY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library