IGVFFI8684DGZI

released

validated

File Set

IGVFDS9907QGGC(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF2-BinD-PCR2_S70

md5sum

e245e4e6a5ebf87be7a62b6c7d5c86f8

Content MD5sum

c3a1b210f9f0606181714d09ce8dff40

File Size

8.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF2-BinD-PCR2_S70_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CTATGCGT:CTCT

Average Read Length

Read Count

370041

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8586BMQG

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library