IGVFFI8664EIWJ

released

validated

File Set

IGVFDS9665KRGJ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF4-BinB-PCR1_S86

md5sum

c49a7d0d6dbb6516a26689dc4dd6f06b

Content MD5sum

4a1bc19587596c71ee1dee24ec924996

File Size

7.0 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF4-BinB-PCR1_S86_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CGCTATGT:CTCT

Average Read Length

Read Count

289796

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5182LFZS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library