IGVFFI8656RWXA

released

validated

File Set

IGVFDS2251KSGP(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR3_S109

md5sum

1114e18c10131293e1cc585e2b615285

Content MD5sum

53414741173d800c8d8fab21f7815bd1

File Size

39 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinA-PCR3_S109_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AACTTGAC:ATTG

Average Read Length

Read Count

1642755

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8346JZPG

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library