IGVFFI8646GHGN

released

validated

File Set

IGVFDS4721YEEW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF2-BinE-PCR4-Rep1_S77

md5sum

b1bdf3cffc8aa2fa7b22e70be995ba32

Content MD5sum

3df351970bae4ba659bd42d2d017c2d8

File Size

20 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF2-BinE-PCR4-Rep1_S77_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTACCAT:GCCA

Average Read Length

Read Count

919013

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9097DRIA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library