IGVFFI8622TDNM

released

validated

File Set

IGVFDS6870QBVM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF2-BinC-PCR4-Rep1_S75

md5sum

a85d5647667ddb32abaed42b53d5eb69

Content MD5sum

1f0b942bb01a279a94315c0ab76ebe00

File Size

19 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF2-BinC-PCR4-Rep1_S75_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TGTCGGAT:GCCA

Average Read Length

Read Count

856938

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8438GPDC

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library