IGVFFI8620KXPV

released

validated

File Set

IGVFDS6296UIVP(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep2_S97

md5sum

a686f9705ea06767bf125f71fd0e9d26

Content MD5sum

e16f77c34e0f33ecda778000382c3924

File Size

19 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinF-PCR3-Rep2_S97_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGGTTATC:TCTC

Average Read Length

Read Count

1001753

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9724UBWS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library