IGVFFI8597VYJU

released

validated

File Set

IGVFDS5675MHUS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR1-Rep1_S124

md5sum

4ffc1debe10e53d1a279a1b699807543

Content MD5sum

d142d1b2ea93217f60b73ce3417d7932

File Size

24 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinD-PCR1-Rep1_S124_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTATGCC:CCAG

Average Read Length

Read Count

1093953

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8987EBZC

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library