IGVFFI8552NKOB

released

validated

File Set

IGVFDS7634SPOA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinD-PCR2_Rep2_S65

md5sum

cff17ab7317ed49b39d86c5fbd22ce58

Content MD5sum

7ada203e9d3653756114dcceca760c8b

File Size

29 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinD-PCR2-Rep2_S65_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GGTCCAGA:TCTC

Average Read Length

Read Count

1464802

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0111NTPA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library