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INDEXED
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SequenceFile
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IGVFFI8529FWOJ
IGVFFI8529FWOJ
preview
validated
Json view
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS5931MYJN
(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW266_1_I2
md5sum
1f0bbe2ac06a546e8e5d09a32eb5303b
Content MD5sum
df40b13ae41542ae6c7a417309cfc55d
File Size
562 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-07-13-McDermott-NextSeq.10X.run3.transcriptome/2023-07-13/outs/fastq_path/AAC5GH5HV/LW266_S6_L001_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Sequencing Details
Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC5GH5HV
Lane
1
Illumina Read Type
I2
Average Read Length
10
Read Count
81170309
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM4930FDUF
InVitroSystem
GM25256 (WTC-11)
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Files Derived From This File
2 items
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
IGVFFI0468AHGV
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
5.2 GB
validated
IGVFFI6973SBDI
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
443 MB
validated
Attribution
Award
HG011996
Principal Investigator(s)
Bruce Posner
,
Gary Hon
,
William Kraus
,
Nikhil Munshi
,
Maria Chahrour
Contact P.I.
Gary Hon
Lab
Gary Hon, UT