Home

IGVFFI8519BPYB

released
validated
File Set
IGVFDS5416ECKJ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinC-PCRrep2_I2
md5sum
0aff771e3fcd1c8f9f7a7caee03880b3
Content MD5sum
477af9f5b061ac15503cd344c1be9135
File Size
14 MB
Submitted File Name
220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-2-BinC_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
GACCAGGA:TGAC
Average Read Length
4
Read Count
1949301

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford