{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI8518KWMY/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI8518KWMY",
"aliases": [
"charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-low-bin-rep2-R1"
],
"assay_titles": [
"CRISPR FACS screen"
],
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "5c9c87f232c4d01dd206c70c79c388ed",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-11-08T20:29:30.847332+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS3348HSLB/",
"accession": "IGVFDS3348HSLB",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/primary-cells/IGVFSM2624KTRK/",
"accession": "IGVFSM2624KTRK",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes"
},
"file_size": 695894509,
"flowcell_id": "AACJJ7LM5",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI8518KWMY/@@download/IGVFFI8518KWMY.fastq.gz",
"illumina_read_type": "R1",
"input_file_for": [
"/alignment-files/IGVFFI4092OXMN/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"lane": 1,
"loci_list_for": [],
"maximum_read_length": 21,
"md5sum": "6b55d7305f4a99a1b0ffa57c1de6fccb",
"mean_read_length": 21,
"minimum_read_length": 21,
"read_count": 39508748,
"release_timestamp": "2024-11-18T21:09:11.185009+00:00",
"s3_uri": "s3://igvf-files/2023/11/08/3e483f0c-2554-4506-bc71-8e2a6568348c/IGVFFI8518KWMY.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "MiSeq Reagent Kit v2",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0004205/",
"status": "released",
"term_name": "Illumina MiSeq"
},
"sequencing_run": 2,
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM73_L3_S3_L001_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 2",
"upload_status": "validated",
"uuid": "3e483f0c-2554-4506-bc71-8e2a6568348c"
}