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IGVFFI8475QQEU

released
validated
File Set
IGVFDS4371VPAL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep2-BinD-PCRrep2_I1
md5sum
be1706427adf8c347838b8ab8df95415
Content MD5sum
823881752e4b1e7dc8e476e5acab8fda
File Size
15 MB
Submitted File Name
220228pPPIF-FFSample-3-D21-pPPIF-Amp-pPPIF-1-2-2-BinD_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
AGCATGGA:TGAC
Average Read Length
8
Read Count
1956760

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford