IGVFFI8461PESG

released

validated

File Set

IGVFDS6281UOJN(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR2_S36

md5sum

7dd7d6906ebccceccc79ab47b58deaf9

Content MD5sum

e89d05fc0592f0ef34a38e124f561a77

File Size

8.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinF-PCR2_S36_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTGAATAG:CTCT

Average Read Length

Read Count

374090

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7141IWGF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library