IGVFFI8442FECN

released

validated

File Set

IGVFDS7080OXFZ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR3_S101

md5sum

7d175ad8fc6d6703c883e17f57ba7bc1

Content MD5sum

e852d7638aedc19e331a5adfc4aa7160

File Size

45 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF1-BinE-PCR3_S101_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGCAATTC:ATTG

Average Read Length

Read Count

1911225

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8090YJPJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library