IGVFFI8369ZSHJ

released
validated
File Set
IGVFDS7638KDSZ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep2-BinC-PCRrep2_R1
md5sum
0efa4d073bdae0010c80eeab9000d8e2
Content MD5sum
58db8746cf27065848906d4952c44d26
File Size
7.8 MB
Submitted File Name
220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S20-BioRep4-FFRep2-BinC-PCRrep2_S221_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
R1
Index
ATTCTAGG:ACTG
Average Read Length
150
Read Count
410868

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution