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IGVFFI8252ZJKQ

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validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2755YZNI(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep1-BinA-PCRrep1_R1
md5sum
7523d5cb8bd00f2d0a1cc4aa8cfacb7c
Content MD5sum
f89f883d93876a70657f69d55bda355b
File Size
25 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_pool-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinA-PCRrep1_S707_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
R1
Index
CTACCAGG:GATATCGA
Average Read Length
151
Read Count
1129694

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
pending

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford