IGVFFI8214NGYS

released

validated

File Set

IGVFDS2059NFJA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96

md5sum

1ed61a71dbcf668017b1d994f31088fe

Content MD5sum

6735efb4fb44bef9a11d15d1bede528c

File Size

15 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinE-PCR3-Rep2_S96_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCCTTGGT:TCTC

Average Read Length

Read Count

789587

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1124JJAA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library