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IGVFFI8170ZADS

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS0561TMHL(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCCA-THP1-BioRep2-FFrep1-BinC-PCRrep4_I2
md5sum
1dc52f8e226164a9a39ae045b5cb8681
Content MD5sum
7bddc7e5418d2e9f01a18b4d47b7374d
File Size
3.3 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C10-Amp_PPIF_5splicesite-BioRep2-FFrep1-BinC-PCRrep4_S817_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
I2
Index
TGCAAGTA:CGCAGACG
Average Read Length
8
Read Count
391450

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
hg19 variant effects
Jesse Engreitz, Stanford
274 B
validated